Wenches will saddle up and cycle 54 miles from London to Brighton in aid of good causes in September.
A group of ten Windsor and Maidenhead cyclists will take on the ride to benefit local and national charities, as each woman will be peddling for a charity close to her heart.
Jayne Starkie, of St Leonard's Road, Windsor, is one of the organisers of The Windsor Wenches. The mum-of-one, who works for BA at Heathrow, said it is the first time she has taken part in such a large fundraising initiative.
Describing herself as a 'leisure cyclist', she said: "A couple of us wanted to do the London to Paris bike ride but it's quite a big thing to do when you've never done one before."
The ladies decided to get together to attempt the shorter trip to Brighton before embarking on a longer journey.
Jayne, 41, added: "We have individual reasons for raising money for different charities. I'm doing it for Breakthrough Breast Cancer because a good friend of mine has breast cancer."
So far she has raised £300 for the cause beating their target of £100 sponsorship each. Her BA colleague Anne Stokes, of Maidenhead, is raising money for Alexander Devine Children's Hospice Service, along with Tobie Butler. Jayne also runs a vintage and designer clothing exchange called Covet Popup with partner and architect Angela Toy who is cycling for Brain Tumour UK.
Natalie Craythorne, of All Saints Avenue, Maidenhead, is fundraising for Multiple Sclerosis Resource Charity and Maria O'Neill, travel manager at Three, is raising money for the Lymphoma Association.
Charity worker Nikki Lowry is supporting the National Autistic Society, Natasha Hill will ride for Action Against Hunger, Helen Lawson is fundraising for baby charity Tommy's and teacher Tonya Tough is supporting Kidney Research UK.
Visit www.doitforcharity.com/jayne, www.doitforcharity.com/tobie, www.doitforcharity.com/mariao, www.doitforcharity.com/hlawson, www.doitforcharity.com/Nikki'sBikeChallenge, www.doitforcharity.com/astokes or www.doitforcharity.com/tonya to donate.
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The Met Office has issued a yellow alert for Windsor and Maidenhead, Slough and Wokingham, warning that rain moving eastwards across the south of the country may turn to snow in some places.