Entries are now open for the return of the Bunny Fun Run on Sunday, March 3.
The event, which is run by the Rotary Club of Maidenhead Thames, attracted about 150 participants on its debut last year despite wet weather.
Organisers are hoping more people will be encouraged to sign up for the 5km race, which starts and finishes in Oaken Grove Park.
"In contrast to the more serious running competitions this is for all the family and is very much a fun run. Anyone can do it," said Rotarian Peter Sands.
"We're hoping it appeals across all sections of the community and it's a chance for everyone to have fun and raise a bit of money."
Children as young as six took part in last year's run, while the oldest competitor was 87.
It takes place on a figure-of-eight-shaped course around the Halifax Road and Belmont areas from 9.30am.
Runners are encouraged to dress up as bunnies and anyone who enters before February 1 will be given a free bunny outfit to wear and keep.
Prizes will be given to the fastest runners as well as the best dressed, but the race is open to all abilities.
Sponsorship money will be split 50/50 between the runner's chosen charity and Rotary causes.
The event is supported by the Michael Shanly Charitable Trust.
Entry costs £15 for 18 and overs, £10 for 12-18s, £5 for six to 12s, or £35 for a family.
Visit www.bunnyfunrun.co.uk for details or to download an entry form.
Road closures will be in place on the day of the run between 9-11.30am. The roads affected are as follows:
Courthouse Road between the junctions with Linden Avenue and St Mark's Road
Belmont Park Road
Belmont Park Avenue
Belmont Road between the junctions with College Road and Hargrave Road
Belmont Vale between the junctions with Cromwell Road and Gordon Road
All Saints Avenue between the junctions with St Mark's Road and Courthouse Road
Halifax Road from the junction with Lancaster Road to north of the junction with Lincoln Road
Lancaster Road between the junctions with Alwyn Road and Halifax Road
Oaken Grove at the junction with Lincoln Road
Vehicular access may also be restricted in the following roads:
Park Side, Elton Drive, Creden Close, Hargrave Road, Grassy Lane, Lynton Green, College Rise, Alexandra Road, Belmont Crescent, Spencers Road, Spencers Close, Cromwell Road, Derwent Drive, Belmont Drive, Mulberry Walk, Ashcroft Road, Murrin Road, Winston Court, Halifax Close, Stirling Grove, Sunderland Road, Hampden Road, Merlin Court, Blenheim Road, Wellington Close, Victor Close, Oldershaw Mews, Taylors Court, Beech Close, Castle Hill Terrace, Loddon Drive, Walker Court, Corfe Place, Archer Close and Langton Close.
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