The Air Ambulance has received a £5,000 donation.
The boost to its finances comes thanks to Nationwide's Big Local Award programme.
Each month Nationwide choose three charities nominated by its members from a different UK region. The public is then allowed to vote online for the charity they would like to get the money.
The Thames Valley and Chiltern Air Ambulance relies on donations to provide its vital service - rescuing injured people from spaces hard for a conventional ground vehicle to reach.
It covers Berkshire, Buckinghamshire and Oxfordshire and secured 43 per cent of the 6,000 votes sent to Nationwide during April.
Sarah Williamson, head of fundraising for the Air Ambulance, said: "For the TVACAA to have gained the majority of votes is very encouraging and we are absolutely delighted with the support we have received. This service relies entirely on charitable donations and these funds will go a long way.’
The Air Ambulance helicopter has flown over 14,000 missions since its inception.
Top Ten Articles
The Met Office has issued a yellow alert for Windsor and Maidenhead, Slough and Wokingham, warning that rain moving eastwards across the south of the country may turn to snow in some places.